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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ORIGINAL RESEARCH Table of Contents   
Year : 2007  |  Volume : 11  |  Issue : 1  |  Page : 15-17
Comparison of three different methods of tissue processing


Department of Oral and Maxillofacial Pathology, Meenakshi Ammal Dental College, Chennai, India

Correspondence Address:
B Sivapathasundharam
Department of Oral and Maxillofacial Pathology, Meenakshi Ammal Dental College, Chennai
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-029X.33958

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Biopsied tissue is cut into thin slices and stained suitably for microscopical examination. Enabling the tissue for sectioning by paraffin embedding is known as tissue processing. The three most commonly employed means of tissue processing are routine manual method, rapid manual method and the microwave method. In this study, sections obtained from the same site of the same tissue were processed by these three methods and stained by hematoxylin and eosin. These sections were then microscopically evaluated by various parameters to compare the three methods. The results that were obtained, after subjecting to statistical analysis, showed no significant differences between the three different processes in terms of quality of staining, clarity of nucleo-cytoplasmic differentiation in various cells and the presence of artifacts. Tissue shrinkage was less in microwave-processed tissue as compared to the other methods. Microwave tissue processing was also found to be more cost-effective than other methods.


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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007