Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contact Us Login 
An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
  Table of Contents    
REVIEW ARTICLE  
Year : 2014  |  Volume : 18  |  Issue : 4  |  Page : 111-116
 

Artefacts in histopathology


Department of Oral and Maxillofacial Pathology, MM College of Dental Sciences and Research, Maharishi Markandeshwar University, Mullana, Haryana, India

Date of Submission10-Oct-2012
Date of Acceptance04-Sep-2014
Date of Web Publication20-Sep-2014

Correspondence Address:
Shailja Chatterjee
Deparment of Oral and Maxillofacial Pathology, MM College of Dental Sciences and Research, Maharishi Markandeshwar University, Mullana - 133 207, Haryana
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-029X.141346

Rights and Permissions

 

   Abstract 

Histopathology is the science of slide analysis for the diagnostic and research purposes. However, sometimes the presence of certain artefacts in a microscopic section can result in misinterpretations leading to diagnostic pitfalls that can result in increased patient morbidity. This article reviews the common artefacts encountered during slide examination alongside the remedial measures which can be undertaken to differentiate between an artefact and tissue constituent.


Keywords: Artefacts, diagnostic pitfalls, histopathology, morbidity, mortality, remedies


How to cite this article:
Chatterjee S. Artefacts in histopathology . J Oral Maxillofac Pathol 2014;18, Suppl S1:111-6

How to cite this URL:
Chatterjee S. Artefacts in histopathology . J Oral Maxillofac Pathol [serial online] 2014 [cited 2022 Jan 28];18, Suppl S1:111-6. Available from: https://www.jomfp.in/text.asp?2014/18/4/111/141346



   Introduction Top


Histopathology is a science completely dependent upon microscopic examination and interpretation. Basic requirements for arriving at a conclusive diagnosis include correct biopsy procedure, proper fixation and processing techniques, adequate sectioning and staining. Identification of structural and morphological details of tissue components is very important for arriving at a conclusive diagnosis. However, sometimes the presence of a foreign substance or alteration in tissue details can create confusion and lead to an incorrect or inconclusive interpretation. Such entities or changes are broadly termed as "artefacts".

An artefact can be defined as an artificial structure or tissue alteration on a prepared microscopic slide as a result of an extraneous factor.


   Causes of artefacts Top


  1. Clinical application of chemicals
  2. Local injection of anesthetics
  3. Surgical suctioning
  4. Excessive heat
  5. Freezing
  6. Surgical mishandling of specimen
  7. Inadequate tissue fixation
  8. Improper fixation medium
  9. Faulty tissue processing
  10. Embedded sponges
  11. Improper staining.



   Classification of Artefacts Top
[1],[2]

  1. During surgery
    1. Injection artefacts
    2. Forceps artefacts
    3. Fulguration artefacts.
  2. During fixation and transport
    1. Fixation artefacts
    2. Freezing artefacts
    3. Artefacts during transportation.
  3. Tissue processing artefacts
  4. Other artefacts
    1. During Surgery.


A. Injection Artefacts

Injection of large amounts of anesthetic solution into the area to be biopsied can produce two major tissue changes: [1],[2]

  1. Needle insertion may produce hemorrhage with extravasation that masks the cellular architecture
  2. Separation of connective tissue bands with vacuolization can occur.



   Remedy Top


Local anesthetic infiltration is acceptable if the field is wide enough in relation to the lesion. Direct injection into the lesion is best avoided.

B. Forceps Artefacts: [1],[2] When the teeth of the instrument penetrate the specimen, it results in voids or tears and compression of the surrounding tissue. The surface epithelium may be forced through the connective tissue producing small "pseudocysts". Compression of the specimen results in loss of cytological details. Nuclear dimensions are especially affected.

Crush artefact: Crush artefact occurs due to tissue distortion resulting from even the most minimal compression of the tissue. It occurs most commonly from mutilation of tissues with forceps during removal, but can be produced by dull scalpel blades that tear the tissue instead of incising it. Crushing produces a destructive and dangerous type of artefact that rearranges tissue morphology and squeezes the chromatin out of nuclei. Inflammatory and tumor cells are most susceptible.


   Remedy Top


  1. Use small atraumatic forceps or Adson's forceps without teeth
  2. A suture should be placed in one edge of the specimen (substitute for forceps for tissue immobilization).


C. Fulguration Artefacts: [1],[2] These artefacts are produced as a result of electrocautery. Heat generated causes marked alteration in both the epithelium and connective tissue. The epithelium and connective tissue get detached and the nuclei assume a spindled, palisading configuration. Separation of epithelium from basement membrane has been observed. The fibrous connective tissue, fat and muscle have an opaque, amorphous appearance. When heat is applied, tissue fluids boil and precipitate protein. Such tissue on microscopic examination shows a coagulated and torn appearance. Alternating zones of coagulation that impart a "Herring bone" appearance can also be seen.


   Remedy Top


  1. The use of electrocautery is contra-indicated as biopsy technique for it is deleterious to interpretation of the tissue although it is desirable for surgery because it aids hemostasis
  2. Care must be exercised to use the cutting and not the coagulation electrode when obtaining a biopsy specimen so that low milliampere current will be produced that will allow efficient cutting and liberation of specimen
  3. The incision margin should be adequaltely away from the interface of the lesion with normal tissue to prevent thermal changes
  4. One should avoid accidental contact of cutting tip with the metallic instrument used for holding the specimen
  5. Scalpel should be used for initial incision around or into the lesion to be biopsied and followed by electrosurgery to complete the removal of tissue.


II. Fixation Artefacts: During fixation, tissues commonly change in volume. The mechanisms involved are ill-understood, but various factors have been suggested, including inhibition of respiration, changes in membrane permeability and changes in ion transport through the membranes. Some intercellular structures such as collagen swell when fixed. Tissues fixed in formaldehyde and embedded in paraffin wax shrink by 33%. The nuclei in frozen sections are usually bigger that those of the same tissue which has been subjected to conventional preparation. Prolonged fixation in formalin can give rise to secondary shrinkage. Hypertonic solutions give rise to cell shrinkage; isotonic and hypotonic fixatives to cellular swelling and poor fixation. The best results, using electron microscopy as the criterion, have been obtained using slightly hypertonic solution (400-450 mOsm; isotonic solutions are 340 mOsm) for fixation.

  1. Artefacts related to diffusion of unfixed material: Diffusion of unfixed material may produce false localization due to movement to some place other than its original location. For example, false localization occurring with glycogen is known as streaming artefact
  2. Diffusion of materials out of tissue: Examples include inorganic ions, cofactors for enzymes, biogenic amines. These have implications in techniques like enzyme histochemistry
  3. False fixation of extraneous material to tissue: This may occur in autoradiography with (H 3 ) labeled amino acids, sugars, thymidine and uridine. Tissues may incorporate these substances into themselves by active metabolism resulting in too high localization of various radioactively labeled substances
  4. Improper Fixation: Delay in fixation or inadequate fixation produces changes like [Figure 1]:
    1. Altered staining quality of cells
    2. Cells appear shrunken and show cytoplasmic clumping
    3. Indistinct nuclear chromatin with nucleoli sometimes not seen
    4. Vascular structures, nerves and glands exhibit loss of detail
    5. Impression of scar formation or loss of cellularity
    6. Use of improper fixative: Fixation in alcohol results in poor staining of the epithelium and improper fixation of the connective tissue. Collagen bundles have an amorphous appearance that is not a result of scar formation but rather result of artefact. Alcohol fixes tissues but causes severe shrinkage. Therefore, alcohol is not recommended as a substitute for formalin except in extreme emergencies. It also makes the tissue brittle, resulting in microtome sectioning artefacts with chattering and a Venetian blind appearance [Figure 2] and [Figure 3]. Currently, 10% neutral buffered formalin is highly recommended for routine fixation purposes. One excellent indicator of poor fixation is the loss of detail of extravasated RBC's.
Figure 1: Photomicrograph showing tissue autolysis due to improper fixation (×10)

Click here to view
Figure 2: Photomicrograph showing folding of section (×10)

Click here to view
Figure 3: Photomicrograph showing contamination by spores (×10)

Click here to view


Fixation artefact simulating acantholytic disease

Tissue fixed in rehydrated formalin exhibits a prominent acantholysis of superficial epithelium with preservation and attachment of the basal cell layer to the underlying tissue. This acantholytic artefact simulates Pemphigus, Hailey-Hailey disease or Darier's disease. A similar artefact is produced by "fixing" the specimen in tap water . [3] Tissues allowed to air-dry will dehydrate, particularly if placed on an adsorbent surface such as gauze sponge. Such tissue cannot be reconstituted and will show dehydration artefact. Other solutions such as saline/saliva are totally inadequate as these substances do not fix tissues and autolysis continues. Curling of tissue during fixation can be prevented if the tissue is laid out on a piece of glazed paper and then fixed (especially applicable to thin mucosal tissues).

Artefacts introduced during specimen transport

I. Freezing Artefacts: These are characterized by formation of interstitial and intracytoplasmic vacuoles resulting from ice-crystal formation taking place as 10% formalin freezes at -11°C. The tissue sections exhibit "Swiss cheese holes" in epithelium and tissue spaces representing areas where forming ice crystals rupture tissue. Sometimes, a granular paranuclear condensation of cytoplasm caused by dehydration of the cells as a result of freezing combined with fixation process is seen.

Remedy

  1. Use of Lillie's acetic acid, alcohol, formalin (AAF): A fixative containing 40% formaldehyde: 10 parts; glacial acetic acid: 15 parts and absolute ethyl alcohol: 85 parts. This solution's freezing point is below -30°C (-22°F).The preservation of morphological details compares favorably with that of tissue fixed in 10% formalin. [3]
  2. Acetic acid causes cellular constituents to swell, thereby, counteracting the adverse effects of alcohol. However, it causes good nuclear preservation.


II. Improper specimen transport, processing and sectioning

During transport, proper measures should be taken for preventing curling of tissue.

Remedy: If the biopsy specimen consists of a small piece of tissue, it is imperative to maintain the epithelium in correct alignment by securing the specimen to a rigid cardboard. Deeper elliptical biopsies usually do not constitute a problem as the various tissue components can be easily identified.

III. Processing artefacts

A. Processing floaters or cutting board metastasis: These are extraneous pieces of tissue contaminating small biopsies. This type of section contamination can take place-

  1. When a biopsy is handled in the laboratory, it may become adulterated with small fragments of other tissues being processed in the same batch
  2. When the tissue sections are being floated out on a water bath they may pick up residual fragments of other sections previously floated out on same water bath. [6] Floater artefact may be suspected if [6]
    1. A tissue fragment looks different from others by virtue of section thickness and/or staining intensity
    2. A tissue fragment is on a slightly different plane from others, especially if superimposed
    3. A tissue fragment showing pathologic changes totally different from others and of a type that one would not have expected at all under the clinical circumstances of the particular case.


Remedy: Use clean cassettes, cutting boards, instruments etc

B. Sponge artefacts [4],[5] : These are seen in tissues placed in cassettes sandwiched between sponges. Angulated holes in the tissue, often triangular with smooth edges are seen at the perimeter. When paired, the angulated holes are occasionally connected by a narrow intervening channel bridging the individual defects. The degree of sponge penetration is related to tissue consistency and is dependent on stromal matrix. For example, soft and lightly fixed specimens generally exhibit greater penetration of sponge bristles than harder well-fixed tissue.

Remedy: Tissue or lens paper is recommended as a substitute.

C. Artefacts of Chemical Treatment:

  1. Most common artefact of this type is "shrinkage". This may be due to dehydration by alcohol. All types of tissue components may appear smaller than they are and clear spaces may appear around such cells and between tissues
  2. Loss of fat: Fat containing cells appear "empty".


IV. Artefacts introduced by microtome [7] :



V. Artefacts of staining and mounting:

  1. Stain Particles: Incorporation of stain particles occurs when staining chemicals are not dissolved properly or the stain is not fresh, leading to precipitation. Remedy: Filter the stain
  2. Air Bubbles: These get trapped when cover slip is placed over stained section. These appear as tiny spherical features.


Remedy

  1. Use adequate amount of mounting media
  2. If the mounting media has attained greater viscosity, xylene can be used as a thinning agent.


VI. Miscellaneous:

  1. Folds in sections: Sometimes wrinkles on the wax sections cannot be straightened resulting in folds or pleats. This is a common artefact in tissues with hard tissue component and is difficult to avoid even with greatest care
  2. Cloudy appearance of sections: Faulty dehydration technique results in cloudiness in sections. Remedy: Rehydrate the tissue section and repeat the processing
  3. Dirt or Dust: Usually the dirt on the slides is "out of focus" when the section is in sharp focus because the cover slip thickness separates the two
  4. Foreign bodies including starch from glove powder entrapped in fibrin and wood fragments from the surface on which tissue is handled and dissected. It is easy to identify these particles of foreign material as artefactual because there will be no associated tissue reaction like foreign body giant cells.


3. Pollen grains contaminating the mountant used (DPX); especially in summer months. These may be misinterpreted as pathogenic fungi but critical examination will reveal that the grains lie outside the plane of section

e. Artefactual pigments

  1. Formalin Pigment: Acid formaldehyde hematin is formed in the tissues by the action of acid formaldehyde solution on hemoglobin. It is therefore found in association with blood and is most commonly seen in spleen, liver, lung, bone marrow and in association with areas of hemorrhage and blood vessels. The pigment appears dark brown in color and is composed of small birefringent crystals. Remedy: Formation of pigment can be limited by fixing in non-acid formaldehyde. For example, neutral buffered formalin. However, formation of formalin pigment is not always prevented by the use of buffered formalin especially, prolonged fixation. Formalin pigment may be removed by treating the section with alcoholic ammonia or alcoholic sodium or potassium hydroxide. These methods, however, have the tendency of removing the sections from the slide. Therefore, the method of choice is use of saturated alcoholic solution of picric acid before the staining procedure. The time required for removal varies from 15 minutes to overnight. Malarial and bilharzial pigments are similar to formalin pigment in all their reactions but may be differentiated from the latter by their intracellular distribution
  2. Mercury pigment: Tissues fixed in solutions containing mercuric chloride contain a varying amount of a dark brown or grey deposit of granules or irregular masses. The deposits are found throughout the tissue. Remedy: It is easily removed from the section by oxidation with iodine to mercuric iodide which can be subsequently removed with sodium thiosulfate
  3. Chromate Deposits: If tissues are not thoroughly washed in water after fixation in chromate-containing fixatives, the chrome salts will react with the dehydrating alcohol forming a yellow-brown to black precipitate within the tissue. Remedy: This can be removed by treating the section for at least 30 minutes with 1% HCl in 70% alcohol.
  4. Stain Deposits: They may be amorphous or crystalline in appearance and are usually highly colored. They are most likely to occur if concentrated solutions are allowed to evaporate on the sections. Remedy: Surface layers of precipitated stain are removed from the staining bath by filtration otherwise the precipitate will be deposited on the surface of section as it is withdrawn from the staining bath.
    • Paraffin Wax: Wax is sometimes retained in the section, particularly in nuclei. It can be clearly recognized by its position, refractile appearance and its birefringence. Remedy: It can be removed by treating the section in xylene at 60°C.


Water: Water droplets in a section may occasionally simulate a light brownpigment. A similar artefact is produced by "fixing" the specimen in tap water . [3]

Other pigments encountered in histological sections are hematoidin (Bright yellow), hemosiderin (light brown), melanin (dark brown) among others.

 
   References Top

1.Margarone JE, Natiella JR, Vaughan CD. Artefacts in oral biopsy specimens. J Oral Maxillofac Surg 1985;43:163-72.  Back to cited text no. 1
[PUBMED]    
2.Ficarra G, McClintock B, Hansen LS. Artefacts created during oral biopsy procedures. J Craniomaxillofac Surg 1987;15:34-7.  Back to cited text no. 2
[PUBMED]    
3.Okun MR, Ellerin P, Piotrowicz MA. Prevention of ice crystal damage to biopsy specimens in transport. Arch Dermatol 1972;105:458-9.  Back to cited text no. 3
[PUBMED]    
4.Kepes JJ, Oswald O. Tissue artefacts caused by sponge in embedded cassettes. Am J Surg Pathol 1991;15:810-2.  Back to cited text no. 4
    
5.Farell DJ, Thompson PJ, Morley AR. Tissue artefacts caused by sponges. J Clin Pathol 1992;45:923-4.  Back to cited text no. 5
    
6.McInnes E. Artefacts in histopathology. Comp Clin Path 2005;13:100-8.  Back to cited text no. 6
    
7.Culing CF, Allison RT, Barr WT. Culling CA in microtome and microtomy. Cellular Pathology Techniques. 4 th ed. Oxford university press.  Back to cited text no. 7
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]


This article has been cited by
1 Deep learning enables ultraviolet photoacoustic microscopy based histological imaging with near real-time virtual staining
Lei Kang, Xiufeng Li, Yan Zhang, Terence T.W. Wong
Photoacoustics. 2022; 25: 100308
[Pubmed] | [DOI]
2 The impact of site-specific digital histology signatures on deep learning model accuracy and bias
Frederick M. Howard, James Dolezal, Sara Kochanny, Jefree Schulte, Heather Chen, Lara Heij, Dezheng Huo, Rita Nanda, Olufunmilayo I. Olopade, Jakob N. Kather, Nicole Cipriani, Robert L. Grossman, Alexander T. Pearson
Nature Communications. 2021; 12(1)
[Pubmed] | [DOI]
3 Processing and Analysis of Tissue Samples from Esophageal Cancer Patients in an African Setting
Lucien Ferndale, Mishalan Moodley, Wenlong C. Chen, Reubina Wadee, Colleen A. Wright, Mohamed Iqbal Parker, Pascale Willem, Christopher G. Mathew
Biopreservation and Biobanking. 2021;
[Pubmed] | [DOI]
4 Kidney Level Lupus Nephritis Classification Using Uncertainty Guided Bayesian Convolutional Neural Networks
Pietro Antonio Cicalese, Aryan Mobiny, Zahed Shahmoradi, Xiongfeng Yi, Chandra Mohan, Hien Van Nguyen
IEEE Journal of Biomedical and Health Informatics. 2021; 25(2): 315
[Pubmed] | [DOI]
5 Deep Learning for Clinical Image Analyses in Oral Squamous Cell Carcinoma
Chui Shan Chu, Nikki P. Lee, Joshua W. K. Ho, Siu-Wai Choi, Peter J. Thomson
JAMA Otolaryngology–Head & Neck Surgery. 2021; 147(10): 893
[Pubmed] | [DOI]
6 Diagnostic concordance between ultrasound-guided core needle biopsy and surgical resection specimens for histological grading of extremity and trunk soft tissue sarcoma
A. Tan, R. Rajakulasingam, A. Saifuddin
Skeletal Radiology. 2021; 50(1): 43
[Pubmed] | [DOI]
7 Comparing Needles and Methods of Endoscopic Ultrasound–Guided Fine-Needle Biopsy to Optimize Specimen Quality and Diagnostic Accuracy for Patients With Pancreatic Masses in a Randomized Trial
Ji Young Bang, Konrad Krall, Nirag Jhala, Charanjeet Singh, Mohamedtaki Tejani, Juan Pablo Arnoletti, Udayakumar Navaneethan, Robert Hawes, Shyam Varadarajulu
Clinical Gastroenterology and Hepatology. 2021; 19(4): 825
[Pubmed] | [DOI]
8 Statistical and machine learning methods for spatially resolved transcriptomics with histology
Jian Hu, Amelia Schroeder, Kyle Coleman, Chixiang Chen, Benjamin J. Auerbach, Mingyao Li
Computational and Structural Biotechnology Journal. 2021; 19: 3829
[Pubmed] | [DOI]
9 Pay attention to doctor–patient dialogues: Multi-modal knowledge graph attention image-text embedding for COVID-19 diagnosis
Wenbo Zheng, Lan Yan, Chao Gou, Zhi-Cheng Zhang, Jun Jason Zhang, Ming Hu, Fei-Yue Wang
Information Fusion. 2021; 75: 168
[Pubmed] | [DOI]
10 Comparative analysis of CT guided vertebral biopsy by a conventional bone biopsy needle versus bone biopsy needle with acquisition cradle
Dharmendra Kumar Singh, Tankeshwar Boruah, Anuradha Sharma, Geetika Khanna, Loveneesh G. Krishna, Nishith Kumar
Journal of Clinical Orthopaedics and Trauma. 2021; 19: 231
[Pubmed] | [DOI]
11 Extreme winter weather: A force to be remembered in dermatopathology
Katie Dreher, Angela Zaladonis, Rodrigo Valdes-Rodriguez
Journal of Cutaneous Pathology. 2021; 48(9): 1204
[Pubmed] | [DOI]
12 Developing a Qualification and Verification Strategy for Digital Tissue Image Analysis in Toxicological Pathology
Aleksandra Zuraw, Michael Staup, Robert Klopfleisch, Famke Aeffner, Danielle Brown, Thomas Westerling-Bui, Daniel Rudmann
Toxicologic Pathology. 2021; 49(4): 773
[Pubmed] | [DOI]
13 Accuracy of cone-beam computed tomography for the evaluation of mandible invasion by oral squamous cell carcinoma
Zezheng Wang, Shuang Zhang, Yumei Pu, Yuxin Wang, Zitong Lin, Zhiyong Wang
BMC Oral Health. 2021; 21(1)
[Pubmed] | [DOI]
14 Detecting Borrelia Spirochetes: A Case Study With Validation Among Autopsy Specimens
Shiva Kumar Goud Gadila, Gorazd Rosoklija, Andrew J. Dwork, Brian A. Fallon, Monica E. Embers
Frontiers in Neurology. 2021; 12
[Pubmed] | [DOI]
15 Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM)
Lukas F. Reissig, Stefan H. Geyer, Julia Rose, Fabrice Prin, Robert Wilson, Dorota Szumska, Antonella Galli, Catherine Tudor, Jacqueline K. White, Tim J. Mohun, Wolfgang J. Weninger
Biomedicines. 2021; 9(11): 1711
[Pubmed] | [DOI]
16 Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Shane M. Hickey, Ben Ung, Christie Bader, Robert Brooks, Joanna Lazniewska, Ian R. D. Johnson, Alexandra Sorvina, Jessica Logan, Carmela Martini, Courtney R. Moore, Litsa Karageorgos, Martin J. Sweetman, Douglas A. Brooks
Cells. 2021; 11(1): 35
[Pubmed] | [DOI]
17 A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System
Lisa Leyssens, Camille Pestiaux, Greet Kerckhofs
International Journal of Molecular Sciences. 2021; 22(6): 3263
[Pubmed] | [DOI]
18 Confocal Laser Microscopy in Neurosurgery: State of the Art of Actual Clinical Applications
Francesco Restelli, Bianca Pollo, Ignazio Gaspare Vetrano, Samuele Cabras, Morgan Broggi, Marco Schiariti, Jacopo Falco, Camilla de Laurentis, Gabriella Raccuia, Paolo Ferroli, Francesco Acerbi
Journal of Clinical Medicine. 2021; 10(9): 2035
[Pubmed] | [DOI]
19 Stress testing pathology models with generated artifacts
NicholasChandler Wang, Jeremy Kaplan, Joonsang Lee, Jeffrey Hodgin, Aaron Udager, Arvind Rao
Journal of Pathology Informatics. 2021; 12(1): 54
[Pubmed] | [DOI]
20 Prevalence of Duodenum Brunner Gland Crush Artifact: A Retrospective Study
Murat ÇELIK
Ahi Evran Medical Journal. 2021;
[Pubmed] | [DOI]
21 Spatially-directed cell migration in acoustically-responsive scaffolds through the controlled delivery of basic fibroblast growth factor
Xiaofang Lu, Hai Jin, Carole Quesada, Easton C. Farrell, Leidan Huang, Mitra Aliabouzar, Oliver D. Kripfgans, J. Brian Fowlkes, Renny T. Franceschi, Andrew J. Putnam, Mario L. Fabiilli
Acta Biomaterialia. 2020; 113: 217
[Pubmed] | [DOI]
22 Quantification of Drugs in Distinctly Separated Ocular Substructures of Albino and Pigmented Rats
Anna-Kaisa Rimpelä, Michel Garneau, Katja S. Baum-Kroker, Tanja Schönberger, Frank Runge, Achim Sauer
Pharmaceutics. 2020; 12(12): 1174
[Pubmed] | [DOI]
23 Challenges of Post-measurement Histology for the Dielectric Characterisation of Heterogeneous Biological Tissues
Alessandra La Gioia, Martin O’Halloran, Emily Porter
Sensors. 2020; 20(11): 3290
[Pubmed] | [DOI]
24 Keratinocyte Dissociation (Desmolysis/Acantholysis) in Ameloblastoma
Sachin C. Sarode, Gargi S. Sarode, Praveen Birur, Yaser A. Alhazmi, Shankargouda Patil
Clinics and Practice. 2020; 10(2): 31
[Pubmed] | [DOI]
25 Ex Vivo Fluorescein-Assisted Confocal Laser Endomicroscopy (CONVIVO® System) in Patients With Glioblastoma: Results From a Prospective Study
Francesco Acerbi, Bianca Pollo, Camilla De Laurentis, Francesco Restelli, Jacopo Falco, Ignazio G. Vetrano, Morgan Broggi, Marco Schiariti, Irene Tramacere, Paolo Ferroli, Francesco DiMeco
Frontiers in Oncology. 2020; 10
[Pubmed] | [DOI]
26 Multiphoton Microscopy of Oral Tissues: Review
Rosa M. Martínez-Ojeda, María D. Pérez-Cárceles, Lavinia C. Ardelean, Stefan G. Stanciu, Juan M. Bueno
Frontiers in Physics. 2020; 8
[Pubmed] | [DOI]
27 THE EYE OF CRAB-EATING FOX (CERDOCYON THOUS): ANATOMICAL CHARACTERISTICS AND NORMATIVE VALUES OF SELECTED DIAGNOSTIC TESTS, MORPHOMETRY OF CORNEAL TISSUE, AND ARRANGEMENTS OF CORNEAL STROMAL COLLAGEN FIBERS
Roberta Renzo, Marcela Aldrovani, Roberta M. Crivelaro, Roberto Thiesen, Alexandre A. F. de Barros Sobrinho, Camila P. Balthazar da Silveira, Amanda P. Garcia, Gabrielle C. S. Campos, Karin Werther, José L. Laus
Journal of Zoo and Wildlife Medicine. 2020; 51(2): 280
[Pubmed] | [DOI]
28 Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography
Neil Marr, Mark Hopkinson, Andrew P. Hibbert, Andrew A. Pitsillides, Chavaunne T. Thorpe
Biological Procedures Online. 2020; 22(1)
[Pubmed] | [DOI]
29 MicroCT optimisation for imaging fascicular anatomy in peripheral nerves
Nicole Thompson, Enrico Ravagli, Svetlana Mastitskaya, Francesco Iacoviello, Kirill Aristovich, Justin Perkins, Paul R Shearing, David Holder
Journal of Neuroscience Methods. 2020; 338: 108652
[Pubmed] | [DOI]
30 Histomorphological assessment of isolated abdominal organs after targeted perfusion with the contrast agent Angiofil® in postmortem computed tomography angiography
Christian Jean-Paul Stumm, Holger Wittig, Nicole M. Kalberer, Eva Scheurer
Forensic Science International. 2020; 315: 110427
[Pubmed] | [DOI]
31 Histological Validation of MRI: A Review of Challenges in Registration of Imaging and Whole-Mount Histopathology
Wadha Alyami, Andre Kyme, Roger Bourne
Journal of Magnetic Resonance Imaging. 2020;
[Pubmed] | [DOI]
32 Revealing the Microscopic Structure of Human Renal Cell Carcinoma in Three Dimensions
S. Ferstl, M. Busse, M. Muller, M. A. Kimm, E. Drecoll, T. Burkner, S. Allner, M. Dierolf, D. Pfeiffer, E. J. Rummeny, W. Weichert, F. Pfeiffer
IEEE Transactions on Medical Imaging. 2020; 39(5): 1494
[Pubmed] | [DOI]
33 Multiscale modelling of drug transport and metabolism in liver spheroids
Joseph A. Leedale, Jonathan A. Kyffin, Amy L. Harding, Helen E. Colley, Craig Murdoch, Parveen Sharma, Dominic P. Williams, Steven D. Webb, Rachel N. Bearon
Interface Focus. 2020; 10(2): 20190041
[Pubmed] | [DOI]
34 Geometry influences inflammatory host cell response and remodeling in tissue-engineered heart valves in-vivo
Sarah E. Motta, Emanuela S. Fioretta, Valentina Lintas, Petra E. Dijkman, Monika Hilbe, Laura Frese, Nikola Cesarovic, Sandra Loerakker, Frank P. T. Baaijens, Volkmar Falk, Simon P. Hoerstrup, Maximilian Y. Emmert
Scientific Reports. 2020; 10(1)
[Pubmed] | [DOI]
35 Utero-placental vascular remodeling during late gestation in Sprague-Dawley rats
Frank T. Spradley, Ying Ge, Joey P. Granger, Alejandro R. Chade
Pregnancy Hypertension. 2020; 20: 36
[Pubmed] | [DOI]
36 Cellular Composition in the Aging Cerebral Cortex of Humans
V. P. Kalanjati, M. W. Hendrata, F. N. Ardana
Neurophysiology. 2019; 51(6): 424
[Pubmed] | [DOI]
37 Renal Papillary Rarefaction: An Artifact Mimicking Papillary Necrosis
John Curtis Seely, Sabine Francke, Steven R. Mog, Kendall S. Frazier, Gordon C. Hard
Toxicologic Pathology. 2019; 47(5): 645
[Pubmed] | [DOI]
38 Nonproliferative and Proliferative Lesions of the Rat and Mouse Hematolymphoid System
Cynthia L. Willard-Mack, Susan A. Elmore, William C. Hall, Johannes Harleman, C. Frieke Kuper, Patricia Losco, Jerold E. Rehg, Christine Rühl-Fehlert, Jerrold M. Ward, Daniel Weinstock, Alys Bradley, Satoru Hosokawa, Gail Pearse, Beth W. Mahler, Ronald A. Herbert, Charlotte M. Keenan
Toxicologic Pathology. 2019; 47(6): 665
[Pubmed] | [DOI]
39 Troubleshooting in Immunohistochemistry with their Remedies
Shankargouda B Patil, Vanishri C Haragannavar, Shwetha K Nambiar
World Journal of Dentistry. 2018; 9(4): 333
[Pubmed] | [DOI]
40 Degradation of Bioresorbable Mg–4Zn–1Sr Intramedullary Pins and Associated Biological Responses in Vitro and in Vivo
Aaron F. Cipriano, Jiajia Lin, Alan Lin, Amy Sallee, Belinda Le, Mayra Celene Cortez Alcaraz, Ren-Guo Guan, Gary Botimer, Serkan Inceoglu, Huinan Liu
ACS Applied Materials & Interfaces. 2017; 9(51): 44332
[Pubmed] | [DOI]



 

Top
Print this article  Email this article
            

    

 
   Search
 
  
    Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
    Article in PDF (948 KB)
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
   Introduction
   Causes of artefacts
    CLASSIFICATION O...
   Remedy
   Remedy
   Remedy
    Classification o...
    References
    Article Figures

 Article Access Statistics
    Viewed8559    
    Printed153    
    Emailed0    
    PDF Downloaded1620    
    Comments [Add]    
    Cited by others 40    

Recommend this journal

© Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007