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| ONLINE ONLY ARTICLES - ORIGINAL ARTICLE |
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| Year : 2022 |
Volume
: 26 | Issue : 2 | Page
: 289 |
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Unveiling the arcanum of formalin-fixed paraffin-embedded archival tissue blocks: A valuable resource for genomic DNA extraction
Sameera Begum1, Vishnudas Prabhu1, Varshasnata Mohanty2, K Krishnaraj Upadhyaya3, Riaz Abdulla1
1 Department of Oral Pathology, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangaluru, Karnataka, India
2 Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru, Karnataka, India
3 Department of General Pathology, Yenepoya Medical College, Yenepoya (Deemed to be University), Mangaluru, Karnataka, India
Correspondence Address:
Riaz Abdulla
Department of Oral Pathology, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangaluru- 575 018, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jomfp.jomfp_424_20
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Background: Formalin-fixed paraffin-embedded (FFPE) tissue blocks are routinely preserved after pathological diagnosis and possess tremendous potential for biomarker discovery. These archival samples are prone to degradation on prolonged storage due to the formalin cross-linking.
Aims: This study aimed to evaluate whether the storage period of the formalin-fixed paraffin-embedded tumor blocks had a significant impact on the yield and purity of the isolated DNA archived for 11 years.
Settings and Design: A retrospective study was carried out in the Department of Oral Pathology and Microbiology in accordance with the Institutional Ethics Committee.
Materials and Methods: Genomic DNA extraction was performed using TaKaRa DEXPAT Easy DNA kit from 40 FFPE tissue blocks of oral squamous cell carcinoma archived for 11 years (2006–2017). NanoDrop spectrophotometer was used to determine the DNA yield (A260) and purity (A260/A280 ratio). The quality of DNA fragments was validated using agarose gel electrophoresis.
Statistical Analysis Used: Statistical analysis was obtained by SPSS 22, MS Excel and analyzed using the analysis of variance (ANOVA) test. P < 0.05 was set for statistical significance.
Results: There was no statistically significant difference observed both in terms of DNA yield (P = 0.996) and purity (P = 0.997) of FFPE tumor blocks archived for 11 years among the study groups.
Conclusions: It was concluded that, irrespective of years of storage of the FFPE, it is possible to extract genomic DNA and use it for molecular studies.
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