Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contact Us Login 
An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ONLINE ONLY ARTICLES - ORIGINAL ARTICLE Table of Contents   
Year : 2022  |  Volume : 26  |  Issue : 3  |  Page : 422
Expression of tyrosinase gene in gingiva: A pilot study


1 Bharati Vidyapeeth Deemed to be University, Dental College and Hospital, Satara Road, Katraj, Dhankawadi, Pune; Department of Periodontics, Dr. GD Pol Foundation's YMT Dental College, Kharghar, Navi Mumbai, India
2 Head of Department of Oral Pathology and Microbiology, Bharati Vidyapeeth Deemed to be University, Dental College and Hospital, Katraj, Dhankawadi, Pune, Maharashtra, India

Correspondence Address:
Rizwan M Sanadi
PhD Student, Bharati Vidyapeeth Deemed to be University, Dental College & Hospital, Satara Road, Katraj, Dhankawadi, Pune & Professor, Department of Periodontics, Dr. GD Pol Foundation's YMT Dental College, Kharghar, Sector - 4, Navi Mumbai, Maharashtra
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jomfp.jomfp_160_22

Rights and Permissions

Background: Melanin is the predominant pigment responsible for the color of skin, hair, iris of eyes, and oral mucosa. Tyrosinase (TYR) is the key enzyme involved in melanin synthesis. Studies in dermatology have shown a positive correlation between TYR enzyme levels and melanin pigmentation of the skin. However, no study has been conducted to assess TYR levels in the gingiva. Hence the present study was conducted to assess TYR levels in gingival melanin hyperpigmentation. Aim: To assess the TYR gene expression in gingiva in individuals with moderate to severe gingival melanin hyperpigmentation. Methodology: Subjects with a chief complaint of blackish appearance of gums with an unesthetic smile were included in the study. Informed consent was obtained. Scaling and root planning were done and subjects were recalled after 2 weeks. The gingival depigmentation procedure was performed using the conventional scalpel technique under adequate local anesthesia. The selected sites underwent conventional gingival depigmentation technique using Bard-Parker handle no: 3 and blade no: 11. The excised layer of epithelium along with a thin layer of underlying connective was sent to the laboratory to assess the TYR gene expression by real-time polymerase chain reaction technique. Results: The levels of the TYR enzyme activity in the gingival tissues from the selected sites were assessed. [Table 1] and [Graph 1] show the levels of TYR enzyme gene expression in the gingival tissue. Conclusion: TYR gene expression and the degree of gingival melanin hyperpigmentation are positively correlated. Hence the assessment of TYR enzyme activity in gingiva could be of great value in today's cosmetologically conscious individuals.


[FULL TEXT] [PDF]*
Print this article  Email this article
    

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed197    
    Printed4    
    Emailed0    
    PDF Downloaded23    
    Comments [Add]    

Recommend this journal

 

Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007