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An Official Publication of the Indian Association of Oral and Maxillofacial Pathologists


 
ORIGINAL ARTICLE Table of Contents   
Year : 2022  |  Volume : 26  |  Issue : 4  |  Page : 603
Exploring a safer alternative to eosin in soft tissue staining


Department of Oral Pathology and Microbiology, MGV's KBH Dental College, Nashik, Maharashtra, India

Correspondence Address:
Surabhi A Sarode
62, Om Sai Nagar, Old Kamptee Raod, Kalamna, Nagpur, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jomfp.jomfp_27_22

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Context: Hematoxylin–eosin (H&E) stain has stood the test of time as the standard stain for histologic examination of human tissues. Haematoxylin is a natural dye, on the contrary, its counterstain eosin is a synthetic dye which belongs to the xanthene group. Synthetic dyes are hazardous to human and animal health. With the increasing awareness of a green earth, it is advisable to use environment-friendly and biodegradable materials. Therefore, an attempt was made to develop as biofriendly substitute in the form of food colour as a counterstain for haematoxylin. Aim: To assess the staining ability of food colouring agents in routine staining and to compare its staining efficacy with Eosin. Settings and Design: Two food colours were obtained and stain was prepared by using 70% ethyl alcohol as counterstain for haematoxylin. Different tissue structures such as epithelium, keratin, collagen fibers, muscles, salivary glands, adipocytes, blood vessels, RBCs were observed and evaluated. Methods and Material: Group A –10 slides stained with green food colour, Group B – 10 slides stained with tomato red food colour and Group C – 10 slides stained with conventional H and E. The stained sections were assessed and graded for nuclear staining, cytoplasmic staining, clarity, uniformity and crispness of staining. Statistical Analysis Used: The non-parametric Kruskal–Wallis and Mann–Whitney U tests were performed for statistical analysis. Results: There was no statistically significant difference between the three study groups with respect to all the parameters except crispness of staining. The crispness of Tomato Red and H and E was better compared to green food colour. Conclusions: Food colouring agents can be used as a safe, biofriendly and inexpensive substitute to eosin in conventional soft tissue staining.


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Journal of Oral and Maxillofacial Pathology | Published by Wolters Kluwer - Medknow
Online since 15th Aug, 2007